Iron-siderophore complexes have high thermodynamic stabilities, so the iron release mechanism from siderophore complexes is not well understood. Our project explores a redox facilitated iron release mechanism from the siderophore, ferrioxamine B. Previous results indicate that iron can be released from ferrioxamine B in the presence of a reducing agent and the iron (II) chelator, BPDS, through the formation of a ternary complex between ferrioxamine B and BPDS. In addition, NADH may be capable of reducing the iron-siderophore complex directly in the presence of low iron (II) chelator concentration. Here we present results on the reaction of ferrioxamine B, NADH and the iron (II) chelator, N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine(TPEN). Preliminary spectroscopic data indicate that NADH is capable of reducing ferrioxamine B in the presence of TPEN. Additionally, TPEN does not form a ternary complex with ferrioxamine B, which is evidence that NADH is capable of directly reducing ferrioxamine B and facilitating iron release.

Reductive mechanism of iron release from the iron-siderophore, ferrioxamine B, by NADH in the presence of the iron (II) chelator, TPEN

245th National Meeting (2013)

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