A fluorophore and analyte (either a PAH or PCB) were bound in gamma-cyclodextrin simultaneously to enable proximity-induced energy transfer. In this system, the analyte acts as an energy donor to the fluorophore, which acts as the energy acceptor. Excitation of the analyte thus results in energy transfer to and emission from the fluorophore. Various PAHs (anthracene, pyrene, phenanthrene, and fluorene) and a variety of di- and tri-substituted PCBs were tested with one of two fluorophores, either a BODIPY derivative or a squaraine derivative. Results indicate that ternary complexes form within the cavity of gamma-cyclodextrin, where both the analyte and fluorophore are bound in the cavity simultaneously. Energy transfer between the analyte and fluorophore has been achieved and a preliminary assay for the array-based detection of such structures has been developed. Further work within this area will lead to the development of fluorescence-based sensors for these carcinogenic and toxic materials.

Array-based detection of carcinogenic polycyclic aromatic hydrocarbons (PAHs) and toxic polychlorinated biphenyls (PCBs)

245th National Meeting (2013)

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