The complex nature of the proteome requires separation methods capable of separating a large number of proteins in order to permit comprehensive analyses. Dynamic IEF is a new method related to capillary isoelectric focusing that can provide excellent resolution and separation capabilities by controlling the shape of the electric field using additional high voltage power supplies. Both the location and width of the focused protein bands can be controlled by manipulation of the electric field.            Current research utilizing dynamic IEF focuses on automating the system using an integrated switching valve to remove separated hemoglobin variants. This analysis demonstrates the exceptional fractionation capabilities of dynamic IEF, as well as the speed of analysis and ease of operation. The ability to automate dynamic IEF to a second dimension helps to show the advantages of using dynamic IEF over traditional IEF and other proteomic separation techniques.

Automated sample extraction using dynamic IEF for the separation and analysis of hemoglobin variants

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.