The success of a robust bioprocess relies on the ability to accurately and effectively control critical process parameters. In the field of single-use bioreactors (SUBR) these evaluations still remain rare and proper control strategies are not clearly outlined. As a result, this study was aimed to provide a rigorous understanding towards developing methods for optimal O2 supply, lower dCO2 built up and establishing a pH control strategy in large scale SUBR.As a first step, mass transfer capabilities were studied using the static gassing out method for determining the volumetric mass transfer coefficient (kLa) for O2 by varying the size and number of microspargers. CO2 cascade feedback strategies (microspargers vs drilled holes) were also evaluated and optimized for the cell culture studies. Following the optimization of the aforementioned physical characteristics, two CHO cell line based 200L fed-batch processes, one with minimal and the other with improved control strategies, were conducted. This study successfully demonstrated the advantages of improved pO2, pH and pCO2 control in a SUBR system.

Establishing improved O<sub>2</sub> supply, lower dCO<sub>2</sub> built up and pH control in large scale single-use bioreactors

245th National Meeting (2013)

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Establishing improved O2 supply, lower dCO2 built up and pH control in large scale single-use bioreactors

Establishing improved O₂ supply, lower dCO₂ built up and pH control in large scale single-use bioreactors