Enzymes are dynamic molecules which undergo multiple conformational changes in the solution, and the dynamics is considered to play a critical role in enzyme activity. Hinge bending motions are known to be related with turnover rate, which result from relative movements of dynamical quasi-rigid bodies and the motions are affected by physical properties of the hinge region. In this study, hinge identification and flexibility modification of that region by mutagenesis were conducted to explore the relationship between the hinge flexibility and the activity. Bacillus circulans xylanase was used to identify the hinge region and the target sites were selected in the region for mutagenesis. As a result, turnover rate (Vmax) was improved. This study indicates the importance of hinge flexibility. The details will be explained and discussed.

Activity enhancement of <i>Bacillus circulans</i> xylanase by modulating flexibility of hinge region

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Activity enhancement of Bacillus circulans xylanase by modulating flexibility of hinge region