Metallic nanoparticles have many potential medical applications, which require specific cell targeting. This can be achieved through protein crosslinking to localize nanoparticles into specific biochemical pathways. In this project, we demonstrate that gold nanoparticles of various ligand shell compositions can be linked to a green fluorescent and glutathione-S-transferase fusion protein. This is done by the use of a homobifunctional amine-reactive cross-linker (DSS) and a heterobifunctional carboxyl-to-amine cross-linker (EDC) to glutathione (GSH). Glutathione will then localize the linked nanoparticle to the GST portion of the fusion protein. The nanoparticle-protein construct is analyzed by high performance liquid chromatography diode array detection, dynamic light scattering of the nanoparticle-protein conjugate, and electron microscopy.

Crosslinking proteins to gold nanoparticles

245th National Meeting (2013)

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