This poster describes the methods and design of the synthesis of precursors (3',4''-dichlorobenztropine and its demethylated analog), as well as the concepts leading up to the synthesis of a final fluorescent molecule. This fluorescent molecule can be used as an alternative to radioactivity in traditional dopamine transporter (DAT) binding affinity assays. The 3',4''-dichlorobenztropineprecursor was selected as  a template due to its high affinity binding and selectivity at the site of the DAT. The free amine of the demethylated benztropine analogallows for the attachment of a small, potent fluorophore via a linker chain. The development of precursors to a fluorescently labeled molecule to be used in DAT binding affinity assays allows for a decrease in both hazard and cost in the process of studying the structure and function of the DAT, potentially increasing DAT-related research output.

Synthesis of the precursors of fluorescence labeled analogs in dopamine transporter binding affinity assays

245th National Meeting (2013)

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