Herein we report the microwave synthesis of fluorescein and fluorescein derivatives, such as carboxyfluorescein and acetylfluorescein, under similar reaction conditions.   These molecules were synthesized and analyzed by second semester organic chemistry students.  Typical student yields and spectra will be presented.  Progression from synthesis of these molecules to utilization of these materials in other laboratories provides an interdisciplinary experience for the students.  For example, acetylfluorescein as well as other esterified derivatives will be used in a biochemistry laboratory where students study the activity of a lipase.  The carboxyfluorescein derivative may be coupled to an antibody and used in a molecular biology laboratory.  Progress in the development of such laboratories will be discussed.

Microwave synthesis of fluorescein and selected derivatives of fluorescein

245th National Meeting (2013)

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