Protein phosphorylation is a biologically important process involved in cell signaling and has been implicated in several human diseases. Information about the dynamic phosphorylation/ dephosphorylation state of kinases is limited. One method to overcome this problem is the development of synthetic small molecules that can recognize and detect phosphoproteins of interest using molecular recognition and fluorescence sensing techniques. Thiol-ene conjugate addition reactions were used to covalently attach synthetic and biomolecular elements such as Zn2+-DPA, dithiothreitol and peptides. Diversity in the sensing array is rapidly generated when each of the molecular entities self-assemble in situ to create a library of differential receptors that can discriminate between kinases. A fingerprint was obtained for different relevant MAP kinases studied in vitro and in complex mixtures such as cell lysates.

In-situ generation of a differential sensing array for identification of kinases in cell lysates

245th National Meeting (2013)

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