Copper-67(67Cu) produced at BLIP was evaluated for radiolabeling of proteins with a series of bi-functional ligands (L  ), 2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7,10- tetraacetic acid (SCN-DOTA), S-2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (SCN-NOTA), and the new N1-(4-isothiocyanatobenzyl)-3,6,10,13,16,19- hexaazabicyclo[6.6.6] icosane-1,8-diamine (NCS-SARAR). The SCN-NOTA, SCN-DOTA, SCN-SARAR were conjugated to proteins (P) (  bovine serum albumin (BSA) and human Immunoglobulin-G (IgG)) with L  :P  molar ratios of 1, 10, 50 and 100 in pH 9 buffer for 1 hour at 23oC. The radiolabeling efficiencies of the conjugates with 67Cu and 57Co (external supplier) at pH 7 were determined, and the optimum L:P  ratio was 10. The number of ligands attached to the protein measured using solutions of Cu(II) and Co(II) (doped with 67Cu and 57Co, respectively) was calculated to be ~0.8 for BSA and ~1.7 for IgG. Radiolabeling efficiency of three 67Cu production batches were compared at 1 to 7 days post EOB. The data was used to establish expiry dates for BLIP produced 67Cu.

Comparing protein radiolabelling of the new NCS-SARAR with NCS-NOTA and NCS-DOTA using copper-67 produced at the Brookhaven Linac Isotope Producer (BLIP)

245th National Meeting (2013)

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