8-oxo-2'-deoxyguanosine (OdG) is a prominent DNA lesion produced from the reaction of dG with reactive oxygen species. Because OdG can form stable base pairs to both dC and dA, it can direct the insertion of dATP during replication, leading to a dG ? T transversion. The potential for OdG to cause mutation is dependent on the preference for dCTP or dATP insertion opposite OdG, as well as the ability to extend past the resulting base pairs. The C2-amine and C8-oxygen could play major roles during these reactions since both can lie outside the Watson-Crick cognate base pairs shape and both can form strong interactions, like hydrogen bonds. In order to gain a more general understanding of how the C2-amine and C8-oxygen of OdG affect its mutagenic potential, the incorporation opposite and extension past seven analogues of dG/OdG that vary at C2 and/or C8 were characterized for several DNA polymerases.

Investigations into the mutagenic potential of 8-oxo-2'-deoxyguanosine using nucleotide analogs

245th National Meeting (2013)

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