Herbal medicament (HM), a hexane soluble extract of Curcuma longa rhizomes is a potent anti-stroke agent. HM constitutes of a mixture of about 20-30 compounds, out of which ar-turmerone is a major constituent. A validated stability indicating method has been developed for the estimation of ar-turmerone in HM oil and capsules as per ICH guide lines. Forced degradation studies of ar-turmerone in pure form, bulk HM oil and HM capsule formulation were performed under conditions of hydrolysis, oxidation, photolysis, temperature and humidity. This newly developed method was capable in better resolution of Ar-Turmerone as compared to earlier reported method.  The results indicated that ar-turmerone was found to be susceptible for oxidative, photolytic and heat conditions. An alternate equation has been developed for estimation of a,�-turmerone (another major constituent of HM, the reference standard of which is not very stable after isolation) using calibration curve of ar-turmerone. The details of procedure will be presented during the conference.

High-performance liquid chromatographic method for estimation of marker compounds in HM-oil and its formulation

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.