Eshmuno CPX has been developed as a new tentacle resin with high binding capacities achieving high resolution and high target yields. With the aim of process improvements over an existing purification this resin was applied for capture of a human growth factor (expressed in E. coli as inclusion bodies), a class of molecules with eminent therapeutic potential.High dynamic binding capacity of 40 mg/ml resin was confirmed loading the refold solution with relatively high conductivity of 36 mS/cm at elevated flow rate of 400 cm/h. This was much superior compared to two other resins which also allow higher process flow rates.Eshmuno CPX provided a strong performance in the separation with purities >95 % and yields of 82 to 96 % depending on the operational conditions.The productivity advantage of Eshmuno CPX will be highlighted in a case study.

Enhanced capture for a recombinant growth factor using Eshmuno CPX

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.