Mammalian cells are the preferred choice for production of monoclonal antibodies in the biopharmaceutical industry. Development of robust and high productivity cell culture processes usually requires multiple experiments involving media, feed, and process optimization. The ability to use shake flasks for such experiments provides an increased throughput and saves time, effort, and resources as compared to the benchtop bioreactors. However, obtaining comparable process results between shake flasks and bioreactors can be challenging, especially in the case of cell lines that have a large oxygen uptake requirement and a propensity to produce lactate and reduce culture pH, which can be detrimental to the cells. In this study, we have developed a new shake flask model which shows comparable performance to 3L bioreactors for a high titer cell culture process by incorporating the use of baffled shake flasks to provide sufficient oxygen transfer to the cells and base addition steps to control culture pH.

Shake flask model develpoment for mammalian cell culture processes

245th National Meeting (2013)

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