Multimodal chromatography has proven to be a powerful tool for solving difficult separation problems. To meet the increasing demands for improved process economy a new chromatographic resin based on the ligand used in Capto� MMC and a more rigid base matrix with smaller particle size has been developed. This resin gives improved resolution, yield, and higher binding capacity at higher flow rates.To exemplify the performance a fast and efficient method to separate monomeric MAb from aggregates is presented including screening for optimal binding conditions in 96-well plate format followed by verification in packed column format and optimisation of elution conditions using DoE. The running conditions were then validated in larger scale with satisfactory correspondence to DoE model prediction.

MAb polishing using multimodal chromatography and model supported process design

245th National Meeting (2013)

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