Superoxide (O2-), one of the reactive oxygen species (ROS), is a toxic molecule produced in the mitochondria during aerobic respiration and in the immune system to kill invasive microorganisms.  Given its toxicity, it must be removed to limit unwanted cellular damage.  Superoxide dismutases catalyze the dismutation of superoxide into molecular oxygen and hydrogen peroxide.  The presence of superoxide can be quantified by measuring the hydrogen peroxide produced upon reaction with superoxide dismutase.  In the interest of developing an inexpensive flow cell for determining superoxide levels in biological tissues, an artificial biomimetic MnIII-salen superoxide dismutase was synthesized as previously described and immobilized onto magnetic beads containing amine groups.  The synthesis, loading, and activity of the particles are described.

Solid phase immobilization of a superoxide dismutase mimic

245th National Meeting (2013)

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