We have shown that imaging mass spectrometry using a NanoSIMS can provide high spatial resolution information on the composition of membrane domains in supported lipid bilayers  [Annual Review of Biophysics, 38 , 53-74 (2009)].  By careful calibration, composition analysis can be made quantitative down to a lateral resolution approaching 50 nm.  This method has now been extended to much more complex domains related to canonical raft compositions by isotopic and atom labeling each component in the mixture.  In addition, we have reorganized components in the membrane using hydrodynamic flow and observed collective reorganization/co-localization as a probe of membrane lateral interactions relevant to clustering in cell membranes.

Membrane organization and re-organization probed by imaging mass spectrometry

245th National Meeting (2013)

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