A novel class of in situ polymerizing thiol-acrylate based copolymers synthesized via an amine-catalyzed Michael addition was studied for its potential to be used in critical-sized bone defect repair. The blends of hydroxyapatite (HA) with PETA-co-TMPTMP (PETA) were evaluated in an effort to create a biodegradable, biocompatible, osteogenic for use as a bone tissue adhesive and filler/scaffold. The potential for the HA:PETA (25:75) composite copolymer to serve as a bone adhesive was tested by gluing bone fragments and subjecting the construct to a shear test, which showed mechanical strength of approximately 2.5 MPa. Osteogenesis experiments were conducted with human adipose derived pluripotent cells to determine the ability of the material to serve as an osteoinductive substrate. HA:PCL (20:80) as the control scaffold while the  experimental scaffolds included HA:PETA composites (wt%): 0:100, 15:85, 20:80 and 25:75. HA:PETA solid composites had higher mechanical strength than the HA:PCL scaffolds. The hASC was cultured for 21 days on HA:PETA scaffold in stromal medium, which displayed the greatest number of live cells.  Moreover, hASC loaded to foamed HA:PETA scaffold displayed increased ALP expression and the highest OCN expression after 21 days in culture. Overall, the HA:PETA had higher mechanical and biocompatibility than the HA:PCL control.

Characterization of novel PETA: Hydroxyapatite scaffolds for human adipose-derived stem cells bone tissue

245th National Meeting (2013)

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