Nanoparticles in physiological media are prone to form protein-coronae[1] and agglomerates due to high salt contents and abundant biomolecules. Depending on the nature of colloidal stabilization, particle characteristics may be affected especially with respect to particle size, which plays a crucial role in terms of biodistribution[2] and cellular uptake pathways[3].We demonstrate the impact of size and surface stabilization of silica- and poly(organosiloxane) nanoparticles to agglomeration at physiological conditions, using Dynamic Light Scattering (DLS), Analytical Ultracentrifugation (AUC) and Asymmetrical Flow-Field Flow Fractionation (AF-FFF). Measurements were accomplished in RPMI cell medium in presence of serum proteins, analogue to in vitro testings.We observed amorphous silica NPs to form agglomerates depending on the size of the silica nanoparticles, whereas glycine stabilized poly(organosiloxane) NPs featured a thin protein corona. In contrast, PEGylated poly(organosiloxane) NPs were found to adsorb nearly no protein determined by a lack of change of hydrodynamic radii. [1] S. Tenzer, D. Docter, S. Rosfa, A. Wlodarski, J. Kuharev, A. Rekik, S. K. Knauer, C. Bantz, T. Nawroth, C. Bier, J. Sirirattanapan, W. Mann, L. Treuel, R. Zellner, M. Maskos, H. Schild, R. Stauber, ACS Nano, 2011 , 5 (9),7155.[2] S. Mitragotri, J. Lahann, Nature Materials, 2008 , 8, 15.[3] J. Kasper, M. I. Hermanns, C. Bantz, O. Koshkina, T. Lang, M. Maskos, C. Pohl, R. E. Unger  C. J. Kirkpatrick, Doi: 10.1007/s00204-012-0876-5.

New insights in protein-based nanoparticle agglomeration: Size characterization of nanoparticle-protein agglomerates in biological relevant environments

245th National Meeting (2013)

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