Carbohydrates on human intestinal cell surface are important recognition sites for gut bacteria including commensals, prebiotics, and pathogens. They are also the major components of human milk oligosaccharides. These carbohydrates are often terminated with a galactose, a fucose, or a sialic acid residue. Carbohydrate structures that exist both in human milk and on the surface of human intestinal cells can be the potent candidates as prebiotics and antimicrobials. We have developed efficient one-pot multienzyme systems for the synthesis of complex human milk oligosaccharides including beta1-3- and beta1-4-linked galactosides, alpha1-3-linked fucosides, as well as alpha2-3-, alpha2-6-, and alpha2-8-linked sialosides. The method can also be used for producing non-natural derivatives of human milk oligosaccharides (HMOs). These compounds have been tested as potential prebiotics and antimicrobials.

Chemoenzymatically synthesized human milk oligosaccharides as potential prebiotics and antimicrobials

245th National Meeting (2013)

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