Surface adsorption of two monoclonal antibodies (mAb1 and mAb2), with widely different hydrophobicity and self-association behavior in solution, was examined by quartz crystal microbalance with dissipation monitoring to understand how adsorption and protein self-interactions near the surface are impacted by their intrinsic properties. The dependence of mass and viscoelastic properties of the adsorbed protein layer on the type of surface, presence of a surfactant, protein concentration, and pH were examined. Next, we have evaluated how these interactions can be further complicated by the introduction of silicon oil (commonly found in silicone oil lubricated syringes)

Elucidating a molecular-level understanding of protein self-association behavior and protein surface interactions by utilizing QCM-D and interfacial tension measurements

245th National Meeting (2013)

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