We report on a system based on gold sensors decorated with peptides supported in organic layers for the selective detection of human immunoglobulin G (IgG). A random copolymer composed of primary amines containing segment and antifouling segment, poly (2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl methacrylate) (poly(AMA-co-HEMA) was grafted from initiator-coated gold substrates by surface initiated polymerization, followed by immobilization of acetylated-HWRGWVA peptide with specific binding affinity to IgG via amide chemistry. The developed biosensing platforms were characterized by a series of analyses and the extent of binding and binding selectivity for target IgG molecules as well as the capability to minimize non-specific interactions with other proteins were examined by quartz crystal microgravimetry (QCM) and surface plasmon resonance (SPR). It is found this sensor exhibits specific binding capability of IgG with a detection limit as low as 0.05 mg/mL while maintaining excellent non-specific protein resistance.

Short peptide-conjugated copolymer based biosensor for specific binding of immunoglobulin G

245th National Meeting (2013)

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