Sequential Injection Analysis (SIA), a new generation of the well established Flow Injection Analysis (FIA) technique, provides improvement in solution manipulation and handling at microliter level. Coupling the SIA with monolithic column creates a novel liquid Sequential Injection Chromatography (SIC) system which can be used for low/medium pressure chemical separation.  The SIC has been reported as an effective alternative to high cost High Pressure Liquid Chromatography (HPLC), especially for separation of mixtures of 3-20 components.  Although a longer column usually provides better separation, in this research, the SIC with ultra-short monolithic column (0.5 cm) was explored using standard parabens as model analytes with the objectives of reducing analysis time, purchasing cost, and organic mobile phase consumption.   Results were compared to those obtained from the HPLC. The SIC operational principle, its advantages and limitations, and summarization of some reported works with unique applications will be presented.

Exploring liquid sequential injection chromatography (SIC)

245th National Meeting (2013)

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