Studies have shown male species of marine life are diminishing while female species of marine life are increasing. One possible link to this observation could be the increased concentrations of estradiol in subterranean groundwater and surface flow. Thus, estradiol and its derivatives are currently being investigated to determine if their concentrations in groundwater are detrimental to marine populations. A method was developed using High Performance Liquid Chromatography (HPLC) with a C18 Column to determine if the concentration of estradiol and its derivatives were of any significance. The mobile phase consisted of acetonitrile in water (60:40 v/v). Prior to sample injection, the autosampler needle was purged and flushed with isopropyl alcohol to rid waste from the column. The samples were run through the column at a flow rate of 1 mL/min for 60 minutes using 1.0 mg/L of �-estradiol in ethanol solution as an internal standard.

HPLC method development and detection of estradiol derivatives in subterranean groundwater

245th National Meeting (2013)

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