In liquid chromatography, mobile phase additives can be used to change selectivity, retention, or efficiency. In this work, cholesterol was used as a mobile phase additive. Coating cholesterol on the stationary phase of a C18 column should mimic how it is arranged in the phospholipid bilayer. This study examined the efficiency of a cholesterol-coated C18 column by observing the changes in the van Deemter coefficients with changing amounts of cholesterol coated. Three solutes, benzene, phenol, and acetophenone, were examined using two different water/organic mobile phases, 40% ACN and 50% MeOH. Three different cholesterol coatings were performed: none, medium, and heavy. The longitudinal diffusion coefficient was found to remain constant, while the eddy diffusion and the resistance to mass transfer coefficients increased, with the mass transfer term having the larger increase.

Efficiency of a cholesterol-coated C18 HPLC column

245th National Meeting (2013)

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