Dairy and cattle farms rely upon bovine pregnancy tests to manage the reproductive schedules of their herds, thereby increasing profitability. One current method of diagnosing bovine pregnancy involves the detection of progesterone levels in blood plasma or milk. This is generally accomplished using an enzyme-linked immunosorbent assay (ELISA), though drawbacks such as false-positives and inconvenience of obtaining plasma samples exist. This poster presents the use of HPLC to detect changes in the levels of potential bovine pregnancy markers: �-Estradiol, Estriol, Estrone, and Progesterone, with the aim of developing a convenient and reliable urine-based bovine pregnancy test. Samples of urine from pregnant cows, sterile cows, and suspected-pregnant cows were analyzed for the aforementioned markers. Results of HPLC analysis, as well as future applications of the method will be discussed.

Detection of steroid pregnancy markers in bovine urine by HPLC

245th National Meeting (2013)

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