There is a need for a novel method for studying transcription factor (TF): DNA binding to be developed, addressing the limitations found in existing methods. The focus of this work is to develop such a method using a microfluidic platform. The process utilizes microfluidic devices, inside which hydrogel technology is incorporated for bioimmobilization of anti-his antibodies, which bind to his-tagged TF's electrophoresed through the gel. Fluorescently labeled DNA sequences can then be electrophoresed through the gel for evaluation of binding to the TF. Measuring binding by fluorescence intensity provided a method for comparison from gel to gel; binding of p53 DNA consensus sequences to p53 TF was distinguishable from non-binding to FOXO1 DNA consensus sequences. Preliminary results in p53 binding experiments demonstrate that hydrogel technology used within microfluidic devices has potential as an effective platform within which to evaluate TF: DNA binding.

Evaluating p53 binding to DNA using microfluidic devices

245th National Meeting (2013)

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