LUV (Large, Unilamellar Vesicles) is composed of self-assembled phospholipids as an enclosed bi-layer with diameter ranging c.a. 100 nm or larger. LUV is often used as a model to investigate structures and functions of bio-membrane and further as a vehicle for drug delivery.  On the contrary, sizes of cells in nature are usually larger than 10 micron, and GUV (Giant Unilamellar Vecilces) is often used as a model. The objective of this study is to compare the physico-chemical properties of LUV and GUV to investigate the relevance of LUV utilization as a model of bio-membrane. We have found the translocation of Cell-Penetrating Peptides (CPP) into cytosol decreased when vesicle become smaller. The result indicates the contribution of lower mobility of lipid molecule at small vesicle causing CPP facing resistance to penetrate. Physiological meaning of such vesicle size will be further discussed at the presentation.

Can LUV with submicron size be a good model for bio-membrane?

245th National Meeting (2013)

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