The formation of antibiotic-resistant biofilms on medical indwelling devices poses a critical problem for medical care. Controlling the adhesion and proliferation of bacteria on these surfaces is necessary to design antimicrobial and antibiofilm materials. Acylase and a-amylase are proposed as efficient biofilm inhibitors, according to their ability to degrade the gram-negative bacterial quorum sensing signals and the exopolysaccharide matrix formed by biofilms, respectively. Nanostructured coatings built on medical devices surfaces prone to colonization (i.e. silicone) were produced using layer-by-layer (LbL) assembly technique. By alternate deposition of negatively charged enzymes and positively charged polyethylenimine a 10 bilayer film was assembled on silicone surface, as proven by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM) techniques. The nanostructured surface was found to be stable and bioactive, with improved wettability and antibiofilm properties, suggesting that it can be further applied in medical devices to counteract biofilm formation.

Nanostructured multilayer coatings with antibiofilm activity for biomedical applications

245th National Meeting (2013)

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