In this paper, we reported a simple and fast immobilization of Horseradish peroxidase(HRP) on PANI nanofibers using in situ polymerization. It is found the micelles composed with aniline monomer and HRP can act as the soft templates to form the PANI nanofibers-HRP. HRP could be attached firmly on the PANI without glutaraldehyde which was necessary for the PANI-HRP in the previous literatures. PANI nanofibers-HRP was tested to oxidize the Guaiacum Phenol and Methyl Orange and it was demonstrated that immobilization significantly enhanced the storage stability of the enzyme. When stored at 4?, no obvious activity loss was observed for the PANI nanofibers-HRP after 6 months while only 20% activity of free HRP was maintained at the same conditions. After 6 times of usage, almost 70% of the initial activity of PANI nanofibers-HRP could be still remained.

Synthesis and properties of the nanostructured polyaniline as a support for horseradish peroxidase immobilization

245th National Meeting (2013)

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