Traditional in vitro stem cell differentiation is controlled via chemical stimuli. However, recent reports have indicated that mechanical stimuli can influence differentiation as well. A number of methods were employed to further elucidate the mechanisms by which such stimuli control human mesenchymal stem cell differentiation: nanostructures (Si nanowires), matrix elasticity (PDMS of varying stiffness), and mechanical force (ultrasound). Additionally, novel surface treatments of materials were investigated to determine their ability to enhance stem cell adhesion and proliferation on a variety of novel surfaces. Differentiation was monitored by standard molecular biology methods (immunostaining, qPCR) as well novel methods including atomic force microscopy (AFM) and ATR-FTIR. These novel material/surface treatments exhibited increased rates of cell adhesion and stem cell differentiation when compared to polystyrene plastic controls. When combined with in vitro differentiation monitoring via AFM and ATR-FTIR, this system allows for increased differentiation rates, and increased ease of monitoring differentiation.

Static and dynamic surfaces to control stem cell differentiation

245th National Meeting (2013)

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