An approach will be described for high-throughput patterning of fibronectin using the headgroups of organosilane self-assembled monolayers to achieve spatial selectivity at the nanoscale. By combining particle lithography with organosilane self-assembly, regularly arranged nanostructures can be prepared for immobilizing proteins. For particle lithography, basic bench chemistry steps of mixing, heating, centrifuging and drying were used to rapidly and reproducibly prepare billions of nanostructures with relatively few defects. The organosilane nanopatterns provide a surface template for studies of biological systems, such as for studies of the extracellular matrix protein, fibronectin. The nanopatterns furnish a robust surface platform that can sustain multiple successive measurements with scanning probe microscopy, and may also be useful for mediating surface directed growth of cells. Scanning probe studies will be presented which evaluate the surface conformation of the protein after immobilization. The nanopatterning approach enables fundamental investigations of protein-binding interactions for application in bioassays and biosensor surfaces.

Directed self-assembly of fibronectin using particle lithography with organosilanes

245th National Meeting (2013)

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