Some Clostridia, including C. ljungdahlii and C. carboxidivorans, are capable of producing ethanol and butanol from CO2 and H2. However, the alcohol productivity, yield, and titer from these native strains are low and uneconomical for industrial applications. In contrast, several acetogenic Clostridia can convert CO2 and H2 to acetate with high yield, titer, and productivity comparable to those from glucose as the substrate. A high metabolic flux from CO2 to acetyl-CoA, the immediate precursor for both acetate and ethanol, is the prerequisite for developing a highly productive ethanol and butanol producers.In this work, we engineered homoacetogens for ethanol and n-butanol production by introducing aldehyde/alcohol dehydrogenase and genes in the acetyl-CoA to butyryl-CoA pathway using modular clostridia plasmids. We then knocked out genes (pta and ack) in acetic acid biosynthesis pathway to further enhance ethanol and butanol production. The fermentation kinetics of these engineered mutants and their ability to produce ethanol and butanol from CO2 and H2 were studied and will be presented in this paper.

Engineering acetogenic Clostridia for ethanol and n-butanol production from CO<sub>2</sub>

245th National Meeting (2013)

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Engineering acetogenic Clostridia for ethanol and n-butanol production from CO2

Engineering acetogenic Clostridia for ethanol and n-butanol production from CO₂