Artificial lipid membranes on graphene and its derivatives are attractive sensing platform for biomembranes by applying unique graphene-based techniques such as fluorescence quenching assay and solution-gated FET. We describe the fabrication of planar lipid membranes on graphene oxide (GO) and reduced GO (r-GO) by the vesicle fusion method, and the effect of the degree of the reduction. The GO flakes deposited on SiO2/Si substrates were reduced with the vapor of hydrazine monohydrate. XPS spectra showed that oxygen components decreased with the reduction time and temperature. The vesicle fusion of zwitterionic dioleoylphosphatidylcholine on GO and r-GO resulted in the formation of double lipid bilayers, single bilayer, adhesive vesicles, monolayer, and bilayer/monolayer stacking structures depending of the reduction degree of r-GO. We also describe the results of fluorescence recovery after photobleaching (FRAP) and single fluorescence molecule tracking of the lipid bilayers on GO to evaluate the membrane fluidity.

Supported lipid membranes on graphene oxide and reduced graphene oxide: Effect of the degree of reduction

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.