Mass spectrometers (MS) have long been used for detection when coupled with HPLC. Nearly universal detection of compounds is achieved when MS is combined with UV detection.  The introduction of lower-cost mass spectrometers allows automated flash chromatography to enjoy the advantages of mass directed purification. These include collection of only the desired product utilizing a targeted molecular weight as a trigger. For natural products, known compounds can be identified and ignored during purification allowing the chemist to isolate only those materials with molecular weights that would suggest they are novel. Selection of ionizing agents become critical to successful generation of an unambiguous molecular ion in flash chromatography and is described in this poster. Mass-directed purification of synthesized compounds is also demonstrated.

Information rich flash chromatography I: Mass directed fractionation

245th National Meeting (2013)

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