Although iron is essential for many metabolic functions �free iron� participates in the generation of free radicals through Fenton�s Chemistry resulting in the depletion of antioxidants and driving the body into oxidative stress. Oxidative stress is implicated in the pathogenesis many cardiovascular, cancer and neurodegenerative diseases. This study presents novel iron chelators based on coupling iron-chelating moieties onto chitosan microspheres. In vitro reaction showed successful coupling of the phenolic molecules as confirmed by HPLC-MS and FTIR. The functionalized chitosan microspheres chelated >70 % as compared to the control. Similarly a method able to monitor the level of oxidants, antioxidants and detect the onset of oxidative stress was developed. This method based on enzymatically generated tetramethoxy azobismethylene quinone (TMAMQ) uses two complimentary approaches. In the presence of antioxidants TMAMQ, a deeply purple quinone is reduced to syringaldazine. Therefore the reduction of TMAMQ or production of syringaldazine can be monitored.

Monitoring oxidative stress and synthesis of chitosan microspheres for iron overload therapy

245th National Meeting (2013)

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