Tuberculosis (TB) is second among the leading infectious disease in the world. To address this problem, new types of potent anti-TB phytotherapeutics are needed. Several secondary metabolites of plant origin elicit interesting inhibitory activity against several species of mycobacteria. As part of our continuing effort in the search of anti-TB constituents from Philippine medicinal plants, we have investigated the secondary metabolites of Goniothalamus gitigensis (Annonaceae) for their in vitro inhibitory activity against M. tuberculosis H37Rv. The air-dried leaves of G. gitigensis were extracted with DCM-MeOH to afford a crude extract (GgD) which was further subjected to acid-base extraction to obtain the crude alkaloid extract (GgA). The non-alkaloidal organic layer was further fractionated via polarity-based solvent partitioning to give the petroleum ether sub-extract (GgP), ethyl acetate sub-extract  (GgE) and n-butanol sub-extract (GgB). Sequential silica gel chromatographic purification of the extracts led to the isolation of three styrylpyrones and an alkaloid. Results of the MABA assay showed a moderately strong antitubercular activity for the isquinoline alkaloid with MIC of 16 mg/mL. Our study shows the potential of the endemic Annonaceae plant, Goniothalamus gitigensis as a promising source of antitubercular secondary metabolites.

Phytochemical studies on the antituberculosis constituents of <i>Goniothalamus sibuyanensis</i>

245th National Meeting (2013)

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Phytochemical studies on the antituberculosis constituents of Goniothalamus sibuyanensis