Polyphenols are found in fruits, vegetables, tea, and red wine. People with diets high in polyphenols have lower incidence of degenerative diseases. It is not clear whether the antioxidant properties or other cellular mechanisms are responsible. Artemisia tridentata ssp. tridentata produces high levels and variety of polyphenols. Previous work by our group indicates that sagebrush polyphenols are antioxidants in vitro. In this study, we investigated whether sagebrush polyphenols act as intracellular antioxidants by affecting the level of glutathione (GSH). HepG2 cells treated with sagebrush extract for 24 hours showed an increase in the levels of intracellular GSH. Sagebrush extract was fractionated using reverse phase HPLC and used to treat HepG2 cells. Relatively lipophilic components of the extract had the greatest effect on GSH levels. Retention times of components of the extract were determined. This study suggests that sagebrush polyphenolics are bioactive and could be exploited as dietary or therapeutic supplements.

Intracellular glutathione levels increase in response to polyphenolic extracts from Artemisia tridentata ssp. tridentata in cultured HepG2 cells

245th National Meeting (2013)

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