In conjunction with our identification of gene-based markers for resistance to aflatoxin accumulation in corn by examination of the plant/pathogen/environment interactions study we have developed a sensitive LC/MS-MS single kernel aflatoxin detection method. Maize hybrids with varying aflatoxin resistance were inoculated with either atoxigenic (21882) or toxigenic (3357) strains of A. flavus, in test plots in Mississippi. Ears were inoculated with toxin-producing A. flavus (NRRL 3357) or non-toxin-producing A. flavus (NRRL 21882) using a single-kernel technique at 14 days after pollination (DAP). As a control, ears were inoculated with water. The corn was the collected one day, three days, seven days, fourteen days, or twenty-one days after inoculation. Kernels were flash frozen in liquid nitrogen to preserve tissue for DNA, RNA, protein, and small-molecule extractions. Aflatoxin was extracted using a 70:30 methanol water solution. The extract was filtered and tested for aflatoxin using an Agilent 6460 LC/MS Triple Quad.

Single corn kernel aflatoxin detection

245th National Meeting (2013)

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