In this study, we describe the application of plasmon-enhanced enzymaticreactions (PEER) to two commonly used enzymes, i.e., �-galactosidase (�-Gal)and alkaline phosphatase (AP). Silver Island films (SIFs) were deposited onto3-Aminoproplytriethoxylsilane (APS)-coated glass slides at different loading(low, medium, and high) which were used as plasmonic surface. Theimmobilization of these enzymes onto SIFs was performed using (1) biotin-avidinprotein assay and (2) biotin-poly (ethylene-glycol)-amine. Enzymatic activitieswere assessed using optical absorption spectroscopy. While an avidin assayenhancement was observed for AP on SIFs, no enhancement was observed for �-Galdue to potential inhibition of enzymatic activity. These results indicate thatany enzymatic process that employs AP can be further enhanced by usingSIFs.

Plasmon-enhanced enzymatic reactions 3: Application to β-galactosidase and alkaline phosphatase

245th National Meeting (2013)

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