Encoded Library Technology (ELT) is a part of GSK's integrated Hit ID strategy. ELT involves creation of large combinatorial libraries whose members (sometimes over a billion!) are encoded by a unique combination of DNA tags. Binders to a molecular target are selected from these libraries and identified using next-generation DNA sequencing. We have developed a platform for translating sequence data back to the encoded chemical warhead, detecting features that are enriched in the selection, and summarizing and annotating the selection experiment. Each week our platform processes over 100 million DNA sequences � larger than the entire human genome. Data visualization is integrated into the TIBCO Spotfire platform, allowing scientists to view summaries of the large data sets, determine the most important chemical space, and then drill down to specific results to prioritize compounds for synthesis and assays. We will describe method details and present examples to highlight our analysis and visualization tools.

Encoded Library Technology data analysis: Finding the grain of sand you want without getting a sunburn

245th National Meeting (2013)

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