Small drug molecules that bind directly to DNA are known to influence vital cell functions such as transcription and DNA replication. Cisplatin is a well-studied anti-tumor drug which has been used extensively to fight various forms of cancer. The high rate of cell apoptosis incurred has been attributed to the 1,2 intrastrand adducts formed between DNA and cisplatin. Currently, little is known about how cisplatin interacts with highly packaged DNA that is typically found within cells. We use osmotic stress coupled with x-ray scattering to study fundamental molecular scale interactions in DNA arrays. Inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to monitor unreacted cisplatin concentrations. DNA-DNA forces were measured as a function of cisplatin concentration. Large disruptions of the DNA-DNA spacings are observed with increasing cisplatin. These studies begin to shed light on how cisplatin interacts with condensed DNA.

Cisplatin adduct affects on DNA-DNA interactions

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.