Nonlinear multi-photon laser wave-mixing spectroscopy is presented as a highly sensitive optical absorption-based detection method that offers many potential applications including detection of �-amyloid for early-stage detection of Alzheimer's disease.  Laser wave mixing offers important advantages including zepto-mole level detection sensitivity, small sample requirements, compact portable designs and high spatial resolution.  Unlike the signal in fluorescence methods, the wave-mixing signal is a coherent laser-like beam that can be detected with high efficiency and minimal background.  We could detect �-amyloid in its native form using UV lasers or with labels using visible lasers.  Our laser wave-mixing detector is interfaced to capillary electrophoresis to separate and identify proteins and biomarkers.  Laser wave mixing offers important advantages including better detection sensitivity levels compared to conventional laser-based methods including fluorescence-based methods.  Potential applications include earlier diagnosis of neurodegenerative diseases, more reliable detection of viruses and detection of chem/bio agents with standoff detection capability.

Sensitive detection of biomarkers for neurodegenerative diseases by nonlinear laser wave-mixing spectroscopy

245th National Meeting (2013)

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