We report on a novel soybean trait that confers tolerance to glyphosate, 2,4-D, and glufosinate herbicides. Three herbicide tolerance genes, 2mEPSPS, aad-12 and pat were constructed into a single vector for constitutive expression. The stacked genes were introduced into the soybean line, Maverick via Agrobacterium tumefaciens-mediated transformation. The transgenic T0 plants were self-pollinated for several generations in contained and regulated field nurseries. Herbicide tolerance and molecular characterization were used for selection of events. The lead event was characterized, selected, and bred into elite soybean varieties. This event provided robust tolerance to single, sequential, and tank-mix applications of 2,4-D and glyphosate as well as to glufosinate. The trait will be identified as Enlist E3� soybeans. Enlist E3� soybeans will be used with a proprietary blend of glyphosate and a new 2,4-D choline. This product, Enlist Duo�, contains a number of innovations to reduce spray drift, post-spray volatilization, and odor and for improved handling characteristics. The combination of Enlist E3� and Enlist Duo� will provide soybean growers with more robust weed management options including the exceptional control of glyphosate-resistant and hard-to-control weeds. [�Trademark of The Dow Chemical Company (�Dow�) or an affiliated company of Dow. Components of the Enlist Weed Control System have been submitted for review and registration and have not yet received regulatory approvals in all countries. The information presented is not an offer for sale. Enlist Duo herbicide is not yet registered for sale or use as a component of the Enlist Weed Control System. Always read and follow label directions. �2013 Dow AgroSciences LLC. Enlist E3 soybeans were co-developed by Dow AgroSciences and M.S. Technologies.]

Triple mode of action herbicide tolerance utilizing the Enlist� Weed Control System in soybeans

246th National Meeting (2013)

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