Hormones are used in food-producing animals to increase feed efficiency, to promote animal muscle development, and to improve meat quality. With the growing number of seafood products imported into the United States, analytical methods are needed to measure multiple hormone residues in seafood products. Our laboratory is developing an analytical method suitable for the simultaneous detection of sixteen conjugated hormonal drugs in rainbow trout muscle at 1 ppb. The sixteen hormones include testosterone, cis-testosterone, progesterone, 19�-nortestosterone, 17a-methyltestosterone, trenbolone, melengestrol acetate, megestrol, 17�-estradiol, 17a-estradiol, ethynyl estradiol, zeranol, estriol, dienestrol, diethylstilbestrol, and hexestrol. The method involves overnight incubation with glucuronidase to deconjugate the hormone glucuronides. After deconjugation, the analytes are extracted from fish muscle tissues followed by sample cleanup using a solvent partition and two solid-phase extractions. An atmospheric pressure chemical ionization (APCI) probe coupled with mass spectrometry analysis in a rapid polarity switching mode is used to quantitate both positively and negatively charged hormones. After method validation, we will use the method to assay muscle tissues derived from dosed rainbow trout. By comparing the hormone levels in dosed muscle tissues analyzed with and without enzymatic digestion, we attempt to address the question whether enzymatic digestion is needed for hormone analysis in rainbow trout muscle tissues.

Determination of hormone residues in rainbow trout muscle using liquid chromatography-tandem mass spectrometry

246th National Meeting (2013)

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