Magic-angle spinning NMR (MAS NMR) spectroscopy provides a robust method for investigating the dynamics and structure of biological molecules in solid state. Gold nanoparticles (AuNPs) functionalized with biological molecules such as proteins and peptides have many potential applications in biomedical fields, including in vivo tumor imaging1, clinical diagnosis2, sensing3, and cell targeting4. MAS NMR technique is performed to characterize the interactions of cysteine-terminated peptides such as Ala-Ala-Cys (AAC) and Cys-Ala-Ala (CAA) with AuNPs. Both peptides are synthesized using manual solid phase peptide synthesis. Cysteine, uniformly labeled 13C and 15N, is incorporated at either the C-terminus or N-terminus of each peptide. Average size of 12.4nm of the AuNPs is determined by Atomic Force Microscopy (AFM). The chemical shift obtained from 13C, 15N and 1H MAS NMR of the selectively labeled peptide on AuNPs is used as a verification of the nature of interactions of peptide on AuNPs. Results from 13C NMR suggest that both peptides are chemisorbed on AuNPs via gold-sulfur interaction. 1H NMR shows the Zwitterions structure of CAA indicating the possibilities of hydrogen bond formation between amino and carboxyl group of neighboring peptides.1.           Zhang, Q.; Iwakuma, N.; Delano, M.; Sharma, P.; Wu, C.; McNeil, J.; Grobmyer, S. R.; Jiang, H.,      Optical Society of America: 2008; p BSuE10. 2.           Baptista, P.; Pereira, E.; Eaton, P.; Doria, G.; Miranda, A.; Gomes, I.; Quaresma, P.; Franco, R., Analytical and Bioanalytical Chemistry 2008,  391 (3), 943-950. 3.          Gates, A. T.; Fakayode, S. O.; Lowry, M.; Ganea, G. M.; Murugeshu, A.; Robinson, J. W.; Strongin, R. M.; Warner, I. M., Langmuir 2008,  24 (8), 4107-4113. 4.           De la Fuente, J. M.; Berry, C. C.; Riehle, M. O.; Curtis, A. S. G., Langmuir 2006,  22 (7), 3286-3293.

Solid-state NMR study of small peptides on goldnanopartilces

246th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.