Accurate quantification of bioactive phytochemicals is challenging as they occur in multiple forms (free, conjugated, and bound) thereby exhibiting wide variations in their polarity and solubility.  This presentation will discuss the influence of the sample preparation and extraction procedures on the assay of phenolic compounds from grains, fruits, vegetables, and dietary supplements.  The results showed that phenolic phytochemicals are not uniformly distributed in foods. The yield of the phenolic compounds extracted from food was affected by the techniques and the conditions used for extraction.  Evaluation of optimization of different parameters reveal that particle size, solvent composition, solid-to-solvent ratio, temperature, and hydrolysis conditions (ultrasonic assisted base hydrolysis, microwave assisted base hydrolysis, and pressurized liquid assisted base hydrolysis) had significant influence on the yield of phenolic compounds extracted from different plant matrices.  Optimization of different parameters and better understanding of their distribution will aid in accurate analysis of multiple forms of bioactive phytochemicals derived from plant products.  This will indirectly improve the reliability and reproducibility of various bioactivity studies of phytochemicals present in foods.

Comparison of extraction and hydrolysis techniques and conditions for the assay of free and bound phenolic compounds from foods

246th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.