Mass spectrometry imaging (MSI) utilizing techniques such as secondary-ion mass spectroscopy (SIMS) and matrix assisted laser desorption (MALDI) deliver atomic and molecular imaging of surfaces in vacuo. Ambient ionization techniques such as desorption electrospray ionization (DESI) offer several advantages over these techniques including minimal sample preparation, operation at atmospheric pressure, simpler instrumentation and lower cost for implementation of analysis. Spatial resolution using traditional DESI is generally on the order of 100 �m. Recent advances in DESI technology have improved the spatial resolution and simplified the analysis process by reducing the number of variables used to optimize the analysis conditions. Changing the geometry of the source has improved the signal-to-noise ratio and lowered limits of detection for analytes in tissue and other matrices. Quantitative molecular imaging of surfaces is made possible by careful sample preparation and advanced software which accounts for tissue-specific suppression. In this presentation, we demonstrate quantitative molecular imaging using DESI under ambient conditions of drug dosed animal tissue without adding matrix to the surface.   Calibration curves were established by coating the entire sample with the isotoically labeled internal standard,including serial dilutions of the target analyte deposited onto the surface of the glass slide and imaging the entire surface.

Quantitative molecular imaging using ambient mass spectrometry

246th National Meeting (2013)

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