EnviroLogix' proprietary next generation DNAble v2.0 chemistry can rapidly and specifically amplify both RNA and DNA targets with single base resolution a billion-fold in 5-10 minutes at a single and constant temperature with results rivaling qPCR. Unlike PCR, DNAble can amplify a target sequence from a crude sample preparation. Assays can be adapted from single reaction point of care lateral flow test strips to fluorescent signal based assays that utilize our two, eight, and sixteen test DNAble portable systems. Standard real-time PCR instruments can run both 96 and 384 well tests for the next level of testing. Expansion of capability into ultra high-throughput applications requires unique instrumentation and chemistry solutions. Leveraging the DNAble chemistry, the Douglas Scientific Nexar system optimized for isothermal amplification and Array TapeTM has been successfully developed to include automating sample handling, master mix addition, array sealing, array incubation at a constant temperature, and fluorescence detection in a continuous reel-to-reel process flow to maximize throughput and reduce costs. This system can process over 122,880 samples per 8 hour day. Examples of validated HTP assays using the Nexar system will be presented and include GMO's from soy and maize.

Inline, ultra high-throughput, Array Tape<sup>TM</sup> based end-point nucleic acid detection: Novel DNAble� v2.0 isothermal amplification system

246th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Inline, ultra high-throughput, Array TapeTM based end-point nucleic acid detection: Novel DNAble� v2.0 isothermal amplification system

Inline, ultra high-throughput, Array Tape^TM based end-point nucleic acid detection: Novel DNAble� v2.0 isothermal amplification system