We recently introduced a tripod-shaped compound, which forms monolayers on graphene and allows functionality to be interfaced in a controlled fashion. Tripod monolayers are 1000 times more stable to desorption than simple aromatic groups, and molecular footprint calculations suggest that the tripods adopt the expected upright configuration on the graphene surface. The function and specificity of graphene biosensors rely on assembling active recognition elements on the surface, ideally through noncovalent interactions that preserve its superior electronic properties. Noncovalent-functionalization strategies to attach biomolecules to SLG are therefore of significant interest. The activity and selectivity of proteins whose function relies on a specific tertiary or quaternary structure, including antibodies and enzymes, had not been demonstrated unambiguously. We found that both an anti-E. coli antibody and the lectin protein, Concanavalin A, denature on graphene-functionalized substrates. Both of the biomolecules retain their recognition functions when immobilized on a monolayer of the tripod molecules, pointing the way forward for electroactive biosensors.

Noncovalent functionalization of graphene with biomolecules

246th National Meeting (2013)

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